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 microRNA Profiling
Exiqon is a leader expertised in providing products for microRNA research. Its microRNA microarray, which is designed based on locked nucleic acids (LNA) proprietary technology, is optimal for profiling of microRNAs with superior sensitivity, specificity and reproducibility. The LNA capture probes are intelligently designed and Tm-normalized ensuring all microRNA targets hybridize to the array with equal affinity.
 
In China, KangChen Bio-tech is the only microRNA microarray service provider for Exiqon. We provide complete microRNA microarray service from sample to results. This service is available to both academic and commercial researchers.
 
microRNA Expression profile service provided by Kangchen Biotech
1.   RNA Extraction
2.   Determine the purity and concentration of total RNA with Nanodrop ND1000
      Determine the integrity of total RNA with Agilent’s 2100 BioAnalyzer or Denaturing Gel Electrophoresis
3.   Total RNA directly labeling
4.   Hybridization
5.   Scan, data extraction and preliminary analysis
6.   Complete report including:
  • Scanned image

    partial enlarged array image
  • Raw and normalized data
  • Significantly up/down regulated microRNA list
 
Additional Service:
Bioinformatic analysis including: Scatter Plot, Volcano plot, Cluster
 
Exiqon microRNA Expression Profiling
Sensitivity
  • Detection limit less than 1 attomole
  • microRNA profiling possible from 30 ng total RNA
Specificity
  • Less than 5% cross hybridization
  • Efficient discrimination between closely related microRNA family members
Reproducibility
  • Uniform spot morphology ensures high array quality
  • More than 98% correlation between arrays
Dynamic range
  • More than 4 logs dynamic range
  • Ensures that high- and low-copy number microRNAs are within linear range
Greatest diversity in field
  • Most comprehensive array portfolio available
  • A number of proprietary miRPlus™ capture probes for detection of microRNAs not included in miRBase

Services
microRNA Profiling
microRNA Sequencing Service
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