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 Whole Genome Expression

KangChen Bio-tech provides complete gene expression microarray service from sample to results. This service, which is available to academic and commercial researchers, employs Phalanx’s and Agilent’s complete microarray platform.

Gene Expression service provided by Kangchen Biotech
1.    RNA Extraction
2.    Determine the purity and concentration of total RNA with Nanodrop ND1000
       Determine the integrity of total RNA with Agilent’s 2100 BioAnalyzer or
       Denaturing Gel Electrophoresis
3.    aRNA synthesis and labeling
4.    aRNA fragmentation
5.    Hybridization
6.    Scan, data extraction and preliminary analysis
7.    Complete report including:
       a.  Scanned image
            Agilent scanned array image:
            
            Partial enlarged array image:
           
            Phalanx scanned array image:
           
            Partial enlarged array image:
           
        b.  Scatter plot, MA-plot
          
        c.   Raw data
        d.  Normalized ratio
        e.  p-value if replicate samples
        f.   Significantly up/down regulated gene list
 
Additional Service:
Bioinformatic analysis including: box plot, PCA, Cluster, QT clustering, Volcano plot, ANOVA, GO annotation
 
Phalanx Whole Genome Microarray:
  • PhalanxJet™ Technology, Superior Precision Printing with Zero Contamination
  • PhalanxArray™ Technology, Ultra High-Throughput Printing
  • PhalanxSlide™ Technology, Patented Advance Permeable 3D Surface Chemistry
  • Phalanx Total Quality Assurance Manufacturing

 

Human Whole Genome Microarray

Mouse Whole Genome Microarray

Number of

Total Probes

32,050

31,802

Number of

Genes Probes

30,968

29,922

Number of

Control Probes

1082

1,880

Probe length

60-mer

70-mer

Agilent Gene Expression Microarray:

  • Agilent’s 60-mer oligonucleotide microarrays (frequently updated content, sequence and annotation information available through Agilent)
  • Microarrays manufactured using computer driven inkjet printing process with SurePrint technology
  • Well-tested protocols (direct labeling or single round amplification labeling)
  • Scanning and feature extraction with Agilent’s DNA Microarray Scanner and SureScan technology
  • Data analysis with GeneSpring

Agilent

Design ID

Array format

Genes coverage

Database source

Whole human genome

14850

4 x 44K

~41,000

Goldenpath, Ensembl, Unigene, Human Genome (Build 33), Refseq, GebBank

whole mouse genome

14868

4 x 44K

~41,000

USC mRNA known genes, Natl. Institute on Aging, Genbank, Unigene, Refseq, Ensembl, RIKEN

whole rat genome

14879

4 x 44K

~41,000

Ensembl, UCSC Goldenpath, Unigene, Refseq, Genbank


Services
Whole Genome Expression
Real-time PCR Array
mRNA Tag Profiling Service
mRNA Sequencing Service
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