康成生物LOGO   Login | Reg | Chinese
 
 
 
 
Search
 
 
  Sequencing Service
 
  Microarray Service
 
Home > Services> Content  
 ChIP-chip
 
KangChen Bio-tech provides complete ChIP-on-chip service from sample to results. This service, which is available to academic and commercial researchers, employs Agilent’s and NimbleGen’s complete microarray platform.
 
ChIP-on-chip service provided by Kangchen Biotech
1.  Cross link proteins to DNA using formaldehyde
2.  Sonication to shear chromatin DNA
3.  ChIP and Purification
4.  Amplification using Sigma WGA kit and Purification
5.  Labeling using Cy3/Cy5
6.  Hybridization
7.  Scan, data extraction and preliminary analysis
8.  Complete report including:
  • Scanned image
          Scanned array image:
         
         Partial enlarged array image:
        
 
  • Raw data
  • log2-ratio data
  • Scatter plot, MA-plot
  • Peaks GFF file
 
Agilent ChIP-chip:
  • Agilent’s 60-mer oligonucleotide microarrays (frequently updated content, sequence and annotation information available through Agilent)
  • Microarrays manufactured using computer driven inkjet printing process with SurePrint technology
  • Well-tested protocols (direct labeling or single round amplification labeling)
  • Scanning and feature extraction with Agilent’s DNA Microarray Scanner and SureScan technology
  • Data analysis with GeneSpring
  • Easily access to probe sequence and annotation information

Agilent
Slides format
Resolution
Description
Human Promoter
244K x 2
Coverage: -5.5k to +2.5k region
~25 probes/gene
Spacing ~195bp
UCSC hg17, NCBI Build 35
~17,000 best defined human transcripts
Mouse Promoter
244K x 2
Coverage: -5.5k to +2.5k region
~25 probes/gene
Spacing ~200bp
UCSC mm7, NCBI Build 35
~17,000 best defined mouse transcripts
Human Proximal promoter
4×44K
Coverage: -1k to +0.3k region
~3 probes/gene
Spacing ~500bp
UCSC hg17, NCBI Build35
~17,000 best defined human transcripts

 
NimbleGen ChIP-chip
  • NimbleGen’s 50-75-mer oligonucleotide probes to equalize the melting temperature
  • Microarrays manufactured using photo-mediated synthesis chemistry with its MAS system
  • Feature extraction with NimbleGen’s NimbleScan™ software
  • Graphical representation of your data with NimbleGen’s SignalMap™ data browser

Catalog Number
Design Name
Promoter Upstream Tiling (bp)
Promoter downstream Tiling(bp)
C4226-00-01
HG18 RefSeq promoter
2200
500
C4222-00-01
MM8 RefSeq promoter
2000
500
C4495001-00-01
RN34 RefSeq promoter
2250
500

 

Services
DNA methylation microarray
ChIP-chip
aCGH
ChIP-Sequencing Service
MeDIP-Sequencing Service
回到页首
 
© Copyright 2007 KangChen Bio-tech Inc. All rights reserved.