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 aCGH
aCGH is a powerful microarray-based technique used to identify and characterize DNA copy number variations across the genome.
 
KangChen Bio-tech provides aCGH (microarray-based comparative genomic hybridization) service from sample to results. This service, which is available to academic and commercial researchers, employs Agilent’s and Nimblegen’s complete microarray platform.
 
aCGH service provided by Kangchen Biotech:
1.  Genome DNA  Extraction
2.  Sonication to shear DNA
3.  Labeling using Cy3/Cy5
4.  Hybridization
5.  Scan, data extraction and preliminary analysis
6.  Complete report including:
  • Scanned image
  • Raw data
  • Scatter plot, MA-plot
  • Normalized ratio
  • p-value if replicate samples
Additional Service:
Bioinformatic analysis including: box plot, PCA, Cluster, QT clustering, Volcano plot, ANOVA, GO annotation.
 
Agilent aCGH microarray
  • Agilent’s 60-mer oligonucleotide microarrays (frequently updated content, sequence and annotation information available through Agilent)
  • Microarrays manufactured using computer driven inkjet printing process with SurePrint technology
  • Well-tested protocols (direct labeling or single round amplification labeling)
  • Scanning and feature extraction with Agilent’s DNA Microarray Scanner and SureScan technology
  • Data analysis with GeneSpring
  • Easily access to probe sequence and annotation information
Agilent
Product Number/ Design ID
Resolution
Description
Probes annotation
Human 244A
4411B
6.4kb
five times more coverage than the original 44K format
UCSC hg18(NCBI Build36)
Mouse 244A
4415B
6.4kb
five times more coverage than the original 44K format
UCSC mm8(NCBI Build36)
Rat 244A
4435A
6.0kb
five times more coverage than the original 44K format
UCSC rn4(Baylor HGSC v.3.4)
Human 44K
4426B / 014950
35kb
Probe coverage spans both coding and noncoding regions,  Well-characterized genes represented by 1+ probes, cancer-relevant genes by 2+ probes
UCSC hg18(NCBI Build36)
Mouse 44K
4426B / 015028
35kb
Probe coverage spans both coding and noncoding regions,  Well-characterized genes represented by 1+ probes, cancer-relevant genes by 2+ probes
UCSC mm8(NCBI Build36)
 
Nimblegen aCGH microarray
  • Highest Resolution Analysis of Entire Genomes Captures More Information Than Other Platforms
  • Long Oligonucleotide Probes Ensure the Highest Sensitivity and Precision
  • Unbiased DNA Copy Number Analysis Provides a Complete Picture
  • Most Up-to-date Genome Build
Human Whole-Genome aCGH
Catalog Number
Organism
Design Name
Description
B4366-00-01
Homo sapiens
HG18 WG CGH
Whole Genome Tiling Array
B4254-00-01
Mus musculus
MM8 WG CGH
Whole Genome Tiling Array
B2432001-00-01
Rattus norvegicus
RN34 WG CGH
Whole Genome Tiling Array
 
Homo sapiens Chromosome Specific 385K Catalog Tiling Arrays
Catalog Number
Design Name
Description
Median Probe spacing
B3732001-00-01
HG18 CHR1 FT
Chromsome 1 Tiling Array
531
B3733001-00-01
HG18 CHR2 FT
Chromsome 2 Tiling Array
575
B3734001-00-01
HG18 CHR3 FT
Chromsome 3 Tiling Array
475
B3735001-00-01
HG18 CHR4 FT
Chromsome 4 Tiling Array
450
B3736001-00-01
HG18 CHR5 FT
Chromsome 5 Tiling Array
425
B3737001-00-01
HG18 CHR6 FT
Chromsome 6 Tiling Array
404
B3738001-00-01
HG18 CHR7 FT
Chromsome 7 Tiling Array
365
B3739001-00-01
HG18 CHR8 FT
Chromsome 8 Tiling Array
341
B3740001-00-01
HG18 CHR9 FT
Chromsome 9 Tiling Array
255
B3741001-00-01
HG18 CHR10 FT
Chromsome 10 Tiling Array
305
B3742001-00-01
HG18 CHR11 FT
Chromsome 11 Tiling Array
310
B3743001-00-01
HG18 CHR12 FT
Chromsome 12 Tiling Array
310
B3744001-00-01
HG18 CHR13 FT
Chromsome 13 Tiling Array
225
B3745001-00-01
HG18 CHR14 FT
Chromsome 14 Tiling Array
200
B3746001-00-01
HG18 CHR15 FT
Chromsome 15 Tiling Array
175
B3747001-00-01
HG18 CHR16 FT
Chromsome 16 Tiling Array
165
B3748001-00-01
HG18 CHR17 FT
Chromsome 17 Tiling Array
160
B3749001-00-01
HG18 CHR18 FT
Chromsome 18 Tiling Array
170
B3750001-00-01
HG18 CHR19 FT
Chromsome 19 Tiling Array
105
B3751001-00-01
HG18 CHR20 FT
Chromsome 20 Tiling Array
134
B3752001-00-01
HG18 CHR21 FT
Chromsome 21 Tiling Array
70
B3753001-00-01
HG18 CHR22 FT
Chromsome 22 Tiling Array
65
B3754001-00-01
HG18 CHRX FT
Chromsome X Tiling Array
340
B3755001-00-01
HG18 CHRY FT
Chromsome Y Tiling Array
20
 

Services
DNA methylation microarray
ChIP-chip
aCGH
ChIP-Sequencing Service
MeDIP-Sequencing Service
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