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 ChIP-Sequencing Service
ChIP-sequencing combines Chromatin Immunoprecipitation (ChIP) with next generation sequencing technology to identify and quantify the binding sites of any DNA-associated proteins (such as transcriptional factors, RNA polymerase, histones) on a genome-wide scale with no constrains. KangChen offers integrated ChIP-sequencing service from sequencing library preparation to comprehensive data analysis.
 
Highlights
HighQualityData: Positional precision of mapped binding sites ±50bp
WideDynamicRange: Robust quantification for determining binding specificities of varying strengths
HighSignal-to-NoiseRatio: Lower background than ChIP-chip, no cross hybridization
Genome-WideAnalysis: Identify any binding sites in promoters, enhancers, exons, introns, even in repetitive regions, not limited to array features or candidate sequences
Low Starting Material Requirement: Robust output from as little as 10ng of precious input
 
ChIP-Sequencing Service
1. Sequencing Library Preparation
    Obtain target DNA fragments by ChIP
    Ligat Adapters to DNA fragments
    Size select the library
    Enrich the adapter-modified DNA framents by PCR
2. Cluster Generation
    Attach DNA to surface of the flow cell
    Bridge PCR amplification
3. Sequencing
    Determine the sequencing cycle
    Sequence
    Image processing and base calling
4. Data Analysis
    Raw data generating
    Genomic mapping and annotation
    Enriched region identification (peak finding)
    Custom data analysis
    Data report
   

   


Services
DNA methylation microarray
ChIP-chip
aCGH
ChIP-Sequencing Service
MeDIP-Sequencing Service
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