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 microRNA Sequencing Service
microRNA sequecing based on the next generation sequencing technology allows us to identify and profile all microRNAs from any organism without prior sequence or secondary structure information. KangChen offers integrated microRNA sequencing service from sequencing library preparation to comprehensive data analysis.
 
Highlights
Superior data: Robust microRNA sequencing and quantification
Universal Discovery: No prior sequence or secondary structure information required
Wide Dynamic Range: Up to four million reads of microRNA can be generated in each flow cell channel
Customizable Size Selection: Any microRNA between 20-25 nucleotides can be investigated
 
microRNA Sequencing Service
1. Sequencing Library Preparation
    Isolate microRNA
    Ligate 5' RNA adapter and 3' RNA adapter
    Reverse transcribe and amplify the microRNA ligated with adapters
    Purify the amplified cDNA construct
2. Cluster Generation
    Attach DNA to surface of the flow cell
    Bridge PCR amplification
3. Sequencing
    Determince the sequencing cycle
    Sequence
    Image processing and base calling
4. Data Analysis
    Raw data generating
    Genomic mapping and annotation
    Differential gene expression analysis
    Custom data analysis
    Data report
 
 

Services
microRNA Profiling
microRNA Sequencing Service
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