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 MeDIP-Sequencing Service
MeDIP-sequencing combines methyl-DNA immunoprecipitation (MeDIP) with next-generation sequencing technology to create moderate resolution methylation profiles of DNA methylation state on a genome-wide scale. KangChen offers integrated MeDIP-sequencing service from sequencing library preparation to comprehensive data analysis.
 
Highlights
Genome-Wide Analysis: Whole-genome analysis of DNA methylation patterns, not limited to array features or candidate sequences
High Signal-to-Noise Ratio: Lower background than MeDIP-chip, no cross hybridization
High Resolution: Positional precision of mapped binding sites ±50bp
Wide Dynamic Range: Robust quantification of the methylation state by simply counting the frequency of reads that map to the same region
Low Sample Amount Required: 10ng fragmented, MeDIP enriched DNA
 
MeDIP-Sequencing Service
1. Sequencing Library Preparation
    Obtain methylated DNA fragments by MeDIP
    Ligate Adapters to DNA fragments
    Size select the library
    Enrich the adapter-modified DNA fragments by PCR
2. Cluster Generation
    Attach DNA to surface of the flow cell
     Bridge PCR amplification
3. Sequencing
    Determine the sequencing cycle
    Sequence
    Image processing and base calling
4.  Data analysis
    Raw data generating
    Genomic mapping and annotation
    DNA methylation state analysis (peak finding)
    Custom data analysis
    Data report    

Services
DNA methylation microarray
ChIP-chip
aCGH
ChIP-Sequencing Service
MeDIP-Sequencing Service
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