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 microRNA芯片技术服务
    microRNA(miRNA)属于Small ncRNAs,是单链的短RNA,长度在22nt左右。microRNA是基因表达的主要调节分子。microRNA以序列互补的方式与特异靶mRNA结合,通过降解靶mRNA或抑制其蛋白翻译调控靶基因的表达。microRNA不但在基本的生物过程,如发育、应激反应、代谢和基因组完整性维持等方面有基本而重要的调控作用;在疾病的发生发展全过程中,也发挥重要的调控作用。
    Exiqon公司基于LNA(锁核酸)专利技术开发出的microRNA研究系列技术平台,为完整而系统的研究microRNA提供全面支持,在microRNA研究领域赢得了良好的声誉和权威性的地位,包括microRNA表达谱芯片,microRNA PCR芯片,microRNA PCR引物,ISH探针,Northern blotting探针,microRNA inhibitor等等。尤其是LNATM microRNA表达谱芯片,使用了基于LNA™专利技术的捕获探针,表现出同类产品无可比拟的优异品质,在microRNA表达分析领域具有国际领先地位。多年来,全球生物学家使用Exiqon miRCURY LNA™芯片发表了大量文章。
    康成独家提供Exqion microRNA芯片全程技术服务已超过十年,实验系统稳定可靠,并且拥有丰富的实验经验,采用康成技术服务发表的SCI论文已超过100篇,平均影响因子在5.5分以上。

康成生物为您提供miRNA表达谱芯片技术服务,您只需要提供保存完好的组织或细胞标本,康成的芯片技术服务人员就可为您完成全部实验操作,包括:RNA抽提,QC,RNA标记,芯片杂交,数据分析等,并提供完整的实验报告。同时,根据您的研究需要,康成还提供多平台联合分析、分子标志物筛选分析等深入数据分析服务。


Exiqon
公司microRNA芯片

芯片名称
种属
检测数目
Database source
miRCURY LNA™ microRNA Array, 7th gen - hsa, mmu & rno
人、小鼠、大鼠
3,100
覆盖miRBase 数据库人,小鼠,大鼠三物种全部microRNA;覆盖与上述三物种相关的所有病毒的microRNA
 
Exiqon LNATM microRNA芯片特点
●  Tm值均一化(72℃),所有microRNA杂交温度一致,保证对所有靶点具有相同的亲和活性(图示)

*图释:通过调整探针中LNA碱基的含量,使不同miRNA探针的Tm值都在72度左右
 
●  AT含量高的microRNA得到有效检测

*图释:对不同AT含量的miRNA均能有效检测

●  高特异性,能区分单碱基差异的microRNA

*图释:miRCURY™ LNA芯片的高特异性
 
●  高灵敏度,可以检测普通DNA作为捕获探针的芯片不能检出的微量microRNA

*图释:基于LNA的探针具有极高的灵敏度。可以检测普通DNA捕获探针不能检出的微量microRNAs;新一代microRNA芯片检出极限可达1 amol,起始总RNA量可低至30ng
 
●  杂交温度高(60℃),有效避免非特异性杂交信号

● 
统一标记方法,无序列偏好,标记效率高,无需富集microRNA

● 
实验结果准确,一步法标记探针,无需纯化,减少信号失真

康成生物microRNA芯片技术服务实验流程
1. 样品RNA抽提
    a. 实验对象为组织样品,取适量(50-100mg)新鲜组织样品或正确保存的组织样品,使用 BioPulverizerTM冰冻粉碎组织,加1ml的RNA抽提试剂TRIzol(Invitrogen),使用Mini-Bead-Beater-16匀浆后抽提RNA。
    b. 实验对象为细胞样品,每份样品取1×106~1×107细胞,加1ml的RNA抽提试剂TRIzol(样品为贴壁细胞,每10cm2培养皿TRIzol使用量为1ml),裂解后抽提RNA。
2. RNA 质量检测
    a. 使用Nanodrop测定RNA 在分光光度计260nm 、280nm和230nm的吸收值,以计算浓度并评估纯度。
    b. 用甲醛电泳试剂进行变性琼脂糖凝胶电泳,检测RNA 纯度及完整性。
    c. 提供RNA QC报告。
    注意:用于芯片检测的RNA样品,必须是高质量的,完整的,没有RNase污染(降解的样品不能用于标记和芯片检测),没有基因组污染。
3. 制备荧光标记探针
     采用miRCURY™ Array Power 标记试剂盒,用标记酶将Hy3™或Hy5™荧光基团标记miRNA, 可以得到用于与芯片杂交的荧光探针。
4. 芯片杂交
    在标准条件下使用MAUI杂交仪将标记好的探针和miRCURY™芯片杂交。
5. 图像采集和数据分析
    使用GenePix 4000B芯片扫描仪扫描芯片的荧光强度,并将实验结果转换成数字型数据保存,使用配套软件对原始数据进行分析运算。
6. 提供实验报告—— 包括详细的实验方法和芯片实验数据及图表
    a. 芯片扫描图
    b. 操作说明(含RNA质检报告)
    c. 芯片数据
    数据汇总表格(EXCEL格式,包含探针位置,探针名称,原始信号值,修正信号值,标准化信号值,样品间microRNA表达量的比值)
    差异表达microRNA的数据表格(EXCEL格式,包含表达上调2倍的microRNA和表达下调2倍的microRNA)
    d. 进一步数据分析
    Scatter Plot(主要针对单色重复实验数据进行相关性R值计算)
    分层聚类(进行多个样品实验时,按基因表达相似程度进行聚类,从而将多个样品进行归类)
    若进行重复实验(≥3)则计算CV值、p值,并做Volcano Plot
 
康成生物基本数据分析展示
1. miRNA差异表达数据
    为了便于样品间的比较,我们通过芯片中位值对miRNA芯片原始信号值进行标准化。通过标准化后的信号值计算出每个miRNA在不同样品间(如处理组 vs 对照组)的表达变化(fold change),并通过ttest计算样品间miRNA表达量变化的统计学显著性。那些表达变化倍数在2倍以上,p值小于0.05的miRNA被挑选出来,并定义为显著性差异表达的miRNA。针对多重比较,p值被校正为FDR。客户可以根据倍数变化,p值,FDR等参数对差异表达的miRNA进行排序和筛选。
    适用范围:两个或两只样品间的比较,建议每组样品数目大于或等于3
*图释:样品间差异表达的miRNA数据示例。
 
2.  差异表达miRNA的聚类图
    层次聚类是一种最常见的用于分析表达数据的聚类方法。它可以根据样品中基因的表达水平将样品自动的分组,可以让客户从整体上评估样品间的基因表达差异,以及样品间的关系。左侧的树状图可以反映样品间基因表达模式的关系(图3)。
    适用范围:两组或多组样品的miRNA表达谱分析

*图释:不同样品组之间差异表达miRNA的聚类示例。
 
3.  差异表达miRNA的火山图
    火山图可以对不同样品组之间差异表达的miRNA进行图形化的展示。横轴代表处理组和对照组之间miRNA表达倍数的变化(log2转化),纵轴代表相应的p值(-log10转化)。火山图可以直观的展示miRNA在样品组间的倍数变化与相应的统计学显著性之间的关系(图5)。纵向的绿线分别对应1.5倍的上调(右侧)和下调(左侧),绿色平行线对应0.05的p值。下面火山图中的红色点代表在不同样品组中具有统计学显著性的差异miRNA(图4)。
    适用范围:两组样品间的差异miRNA分析,每组样品数目必须大于或等于3

*图释:火山图示例。
 
康成生物高级数据分析展示
1) 差异表达microRNAs的靶基因预测
    康成生物通过汇总三大数据库(miranda, mirbase, targetscan)的已知miRNA靶点信息, 我们将三种数据库的结果交集做为最终miRNA的靶基因结果,有效的降低了靶基因预测的假阳性率,见下表。Venn图展示了对于特定miRNA列表利用上述数据库的多个预测结果的整合分析。
    适用范围:两组或多组数据比较获得的差异miRNA
*图释:左图:康成生物miRNA靶基因预测结果的Venn图示例;右表:康成生物miRNA靶基因预测结果列表示例。
 
2) microRNA—靶基因网络图
    可以从整体上挖掘miRNA和其靶基因的调控关系, 下面的网络图中红色结点为miRNA,蓝色结点为靶基因,结点大小越大说明这个节点在microRNA—靶基因相互作用网络中的位置越重要。
    适用范围:两组或多组数据比较获得的差异miRNA,一般情况下用于分析的miRNA数目应小于10个。

*图释:康成生物miRNA—靶基因网络图示例。
 
3) 靶基因的功能分析:Go & Pathway分析
    为了获得对microRNAs生物学功能的理解,鉴定其全部靶mRNA(靶基因)是必不可少的。康成使用多种数据库来预测人类已知microRNA靶基因。之后对这些基因的功能进行分析(GO分析和KEGG pathway分析)。

*图释: A. GO分析结果展示:预测的差异表达microRNAs靶基因的前10个富集生物学过程;B. Pathway分析结果展示:预测的microRNA靶基因(CKS1、Skp2等,黄色标注)与细胞周期相关,提示microRNAs可能涉及到小细胞肺癌的发生。
 
联合分析
1) microRNA芯片与lncRNAs芯片结果联合分析(ceRNA Analysis)
    根据ceRNAs(competing endogenous RNAs,竞争性内源RNAs)假说,mRNAs、和长链非编码RNAs(long non-coding RNAs,LncRNAs)间通过使用microRNA响应元件(microRNA response elements,MREs)作为一种“语言”彼此间进行“通信”, 形成一个复杂的调控网络,在病理条件下(例如癌症),发挥着重要的作用。通过联合microRNA表达谱和Arraystar LncRNA芯片结果,能够找出最有可能的ceRNA,并绘制ceRNA调控网络。
    康成生物通过MuTaMe(mutually targeted MRE enrichment )分析寻找我们感兴趣的lncRNA 的ceRNAs,进而构建miRNA、lncRNA和ceRNA的调控网络,从而揭示ceRNA通过竞争性结合miRNA来调控目的lncRNA的新的调控模式
    适用范围:同时具有miRNA表达谱数据和LncRNA芯片数据的样品
 

*图释: ceRNA分析结果展示。左侧:ceRNA网络图。右侧:针对网络图中的特定LncRNA(lincRNA-GPR180)的LncRNA-miRNA-mRNA相互作用详细信息。
 
2) microRNA芯片与mRNA芯片结果联合分析
    根据表达谱芯片结果和miRNA芯片结果联合分析,进一步定位差异miRNA发挥功能的途径以及相应的差异表达靶基因可能引起的生物学通路变化。首先利用差异表达的miRNA找寻靶基因,将其与表达谱中的差异表达基因取交集。这样能找到既是差异miRNA的靶基因又在表达谱芯片中差异表达的基因,可以进一步缩小miRNA可能影响的基因的范围,能够更加准确地研究这些基因所发挥的生物学功能。
    适用范围:同时具有mRNA表达谱数据和miRNA表达谱数据的样品,一般情况下用于分析的差异miRNA数目应小于10个

*图释:康成生物microRNA芯片与mRNA芯片联合分析示例:miRNA-靶基因网络图。
 
3) microRNAs与DNA甲基化芯片结果联合分析
    DNA甲基化和microRNA调控是两种重要的表观遗传现象。研究表明,特定microRNA能够作为肿瘤抑制microRNA靶向DNA甲基转移酶(DNA methyltransferase,DNMT)。例如miR-29家族microRNA靶向DNMT 3A和3B,导致全基因组DNA甲基化的改变。同时,抑癌microRNA的启动子甲基化程度的改变也会影响癌症(例如多发性骨髓瘤)的发生。联合microRNAs和DNA甲基化芯片结果能够研究两者之间的复杂调控关系,更好地理解癌症发生的机制。
    适用范围:同时具有microRNA表达谱数据和甲基化谱数据的样品

康成客户实例——microRNA表达谱芯片
1. microRNA标志物筛选应用实例
筛选与肝移植后的肝癌复发相关的miRNA (Identification of recurrence-related microRNAs in hepatocellular carcinoma following liver transplantation.2013, Molecular Oncology.)
   
肝癌(HCC)患者进行肝移植后,依然可能复发。如果复发,则预后差。目前,预后判断主要采用米兰标准和UCSC标准,这两种方法主要基于肿瘤大小和损伤数目来进行预判,并不能准确预测疾病进展和预后。该研究发现,在复发和不复发的病人之间,microRNA表达谱有明显差异。在显著变化的microRNA中,6个microRNA(miR-19a, miR-886-5p, miR-126, miR-223, miR-24 and miR-147)作为一个整体,可以做肝癌复发和病人生存的预后判断,与现有的预后判断方法,如米兰标准相比较,microRNA分子标记物准确性好,特异性强。研究者对挑选出的microRNA的功能进行了初步的探讨,发现这些microRNA参与了肝癌复发相关的信号通路中,为进一步阐明microRNA在肝癌中的作用机制奠定了基础。

结果展示

*图释:芯片筛选差异表达的microRNA,可以将复发与不复发组区分开。
 
*图释:ROC分析检测microRNA分子标记物的灵敏度和特异性
 
2. microRNA的功能机制研究实例
椎间盘退行性病变患者中的miRNA表达谱特征 (Characterization of microRNA expression profiles in patients with intervertebral disc degeneration. 2014, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE )
    本研究通过microRNA芯片筛选和PCR验证,研究者发现了在腰椎间盘突出症和脊髓损伤中表达有显著差异的microRNA。围绕这些microRNA,通过生物信息学分析预测了microRNA的靶基因和microRNA可能参与的信号通路,包括PI3K-Akt通路,MAPK信号通路, EGFR通路和Wnt信号通路等。本研究初步探讨了microRNA在IDD的发生和发展所发挥的功能。接下来,可以针对这些microRNA和相应的靶基因展开详细的功能研究,为进一步阐明IDD的分子机制,未来开发IDD的诊断试剂盒和治疗靶点选择提供初步的实验依据。
 
技术路线
 
3. microRNA标志物筛选和功能分析联合应用实例
MicroRNA-135a contributes to the development of portal vein tumor thrombus by promoting metastasis in hepatocellular carcinoma. 2011, JHEPAT.)
    本研究通过对人癌组织,肝癌细胞株和小鼠模型的系列实验发现,miR-135a的表达上调,可以促进PVTT转移,而这种作用可能是通过抑制MTSS1(metastasis suppressor 1)而实现的。研究还发现,转录因子FOXM1可以促进miR-135a的表达,激活miR-135a相关的信号通路。从而提出FOXM1-miR-135a-MTSS1通路。研究者进一步选取了50例肝癌和PVTT对照样本,探讨miR-135a的biomarker潜能,发现miR-135a和存在PVTT的肝癌患者的预后和生存有相关性。
 
技术路线
 
结果展示

*图释:差异表达的microRNA的聚类分析(miRNA芯片数据)。
 

*图释:miR-135表达量不同的病人的KM生存线(miRNA qRCR数据)。

国内学者使用康成Exiqon microRNA芯片技术服务发表的SCI平均影响因子5.5分以上
→  Role of microRNA-4516 involved autophagy associated with exposure to fine particulate matter. Oncotarget. 2016. (New)

→  microRNA-802/Rnd3 pathway imposes on carcinogenesis and metastasis of fine particulate matter exposure. Oncotarget. 2016. (New)

→  MicroRNA-1228* inhibit apoptosis in A549 cells exposed to fine particulate matter. Environ Sci Pollut Res. 2016. (New)

→  miRNA expression profile of vulvar squamous cell carcinoma and identification of the oncogenic role of miR-590-5p. Oncol Rep. 2016. (New)

→  Fluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis. PLoS One. 2015.

→  MiR-130a and MiR-374a Function as Novel Regulators of Cisplatin Resistance in Human Ovarian Cancer A2780 Cells. PLoS One. 2015.

→  Association of Altered Serum MicroRNAs with Perihematomal Edema after Acute Intracerebral Hemorrhage. PLoS One. 2015.

→  Micro-vesicles derived from bone marrow stem cells protect the kidney both in vivo and in vitro by microRNA-dependent repairing. Nephrology (Carlton). 2015.

→  Investigation of microRNA expression profiles associated with human alcoholic cardiomyopathy. Cardiology. 2015.

→  MicroRNA-374b Suppresses Proliferation and Promotes Apoptosis in T-cell Lymphoblastic Lymphoma by Repressing AKT1 and Wnt-16. Clin Cancer Res. 2015.

→  Circulating cell-free miRNAs as biomarker for triple-negative breast cancer. Br J Cancer. 2015

→  MicroRNA expression profiles and target prediction in neonatal Wistar rat lungs during the development of bronchopulmonary dysplasia. Int J Mol Med. 2015.

→  Expression of somatostatin receptor subtype 2 in growth hormone-secreting pituitary adenoma and the regulation of miR-185. J Endocrinol Invest. 2015.

→  The expression profiles of microRNAs in Kaposi's sarcoma. Tumour Biol. 2015.

→  Differential expression analysis of miRNA in peripheral blood mononuclear cells of patients with non-segmental vitiligo. J Dermatol. 2015.

→  Identification of differentially expressed microRNAs involved in non-traumatic osteonecrosis through microRNA expression profiling. Gene. 2015.

→  Differentially expressed microRNAs in bone marrow mesenchymal stem cell-derived microvesicles in young and older rats and their effect on tumor growth factor-beta1-mediated epithelial-mesenchymal transition in HK2 cells. Stem Cell Res Ther. 2015.

→  Mertk deficiency alters expression of micrornas in the retinal pigment epithelium cells. Metab Brain Dis. 2015.

→  mRNA and microRNA expression profiles of radioresistant NCI-H520 non-small cell lung cancer cells. Mol Med Rep. 2015.

→  Grouping Pentylenetetrazol-Induced Epileptic Rats According to Memory Impairment and MicroRNA Expression Profiles in the Hippocampus. PLoS One. 2015.

→  Methylation-induced loss of miR-484 in microsatellite-unstable colorectal cancer promotes both viability and IL-8 production via CD137L. J Pathol. 2015.

→  Mycophenolic acid upregulates miR-142-3P/5P and miR-146a in lupus CD4+T cells. Lupus. 2015.

→  MicroRNA-204 targets signal transducer and activator of transcription 5 expression and inhibits proliferation of B-cell lymphoma cells. Mol Med Rep. 2015. 

→  Converging Evidence Implicates the Abnormal MicroRNA System in Schizophrenia. Schizophrenia Bulletin. 2015. 

→  MicroRNA-223-3p Inhibits the Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells via Affecting RPS6KB1/hif-1a Signal Pathway. PLoS One.2015.

→  Microarray analysis revealed markedly differential miRNA expression profiles in cervical intraepithelial neoplasias and invasive squamous cell carcinoma. Future Oncol. 2014.

→  Epigenetic silencing of microRNA-199b-5p is associated with acquired chemoresistance via activation of JAG1-Notch1 signaling in ovarian cancer. Oncotarget. 2014.

→  Identification of Aberrantly Expressed miRNAs in Gastric Cancer. Gastroenterol Res Pract. 2014.

→  Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells. Oncol Rep. 2014.

→  Characterization of microRNA expression profiles in patients with intervertebral disc degeneration.Int J Mol Med. 2014.

→  Characterization of paraquat-induced miRNA profiling response in hNPCs undergoing proliferation. Int J Mol Sci. 2014.

→  Deregulation of serum microRNA expression is associated with cigarette smoking and lung cancer. Biomed Res Int. 2014.

→  Time-specific microRNA changes during spinal motoneuron degeneration in adult rats following unilateral brachial plexus root avulsion: ipsilateral vs. contralateral changes. BMC Neuroscience. 2014.

  MicroRNA Expression Profile and Functional Analysis Reveal that miR-206 is a Critical Novel Gene for the Expression of BDNF Induced by Ketamine. Neuromol Med. 2014.

 Oncogenic miR-20a and miR-106a enhance the invasiveness of human glioma stem cells by directly targeting TIMP-2. Oncogene. 2014.

 MEK inhibitor effective against proliferation in breast cancer cell. Tumor Biology. 2014.

 7-Chlorokynurenic acid (7-CTKA) produces rapid antidepressant-like effects: through regulating hippocampal microRNA expressions involved in TrkB-ERK/Akt signaling pathways in mice exposed to chronic unpredictable mild stress. Psychopharmacology.2014.

 Differential expression of miRNA in rat myocardial tissues under psychological and physical stress. Experimental and Therapeutic Medicine. 2014.

 Altered microRNAs expression in inflamed and non-inflamed terminal ileal mucosa of adult patients with active Crohn's disease. Journal of Gastroenterology and Hepatology. 2014.

 miR-429 Identified by Dynamic Transcriptome Analysis Is a New Candidate Biomarker for Colorectal Cancer Prognosis. OMICS: A Journal of Integrative Biology.2014.

 MiR-124 protects human hepatic L02 cells from H2O2-induced apoptosis by targeting Rab38 gene. Biochemical and Biophysical Research Communications. 2014.

 Deregulated microRNAs in CD4+ T cells from individuals with latent tuberculosis versus active tuberculosis.J Cell Mol Med.2014.

  Special role of Foxp3 for the specifically altered microRNAs in Regulatory T cells of HCC patients.BMC Cancer. 2014.

  miR-1207-5p and miR-1266 suppress gastric cancer growth and invasion by targeting telomerase reverse transcriptase.CELL DEATH & DISEASE.2014.

  MiR-139-5p inhibits HGTD-P and regulates neuronal apoptosis induced by hypoxia–ischemia in neonatal rats.Neurobiology of Disease.2014.

  Deregulated microRNAs in gastric cancer tissue-derived mesenchymal stem cells: novel biomarkers and a mechanism for gastric cancer.Brit J Cancer.2014.

  Identification of latent tuberculosis infection-related microRNAs in human U937 macrophages expressing Mycobacterium tuberculosis Hsp16.3. BMC Microbiology.2014.

  MicroRNA155 in oral squamous cell carcinoma: Overexpression, localization, and prognostic potential.HEAD NECK-J SCI SPEC.2014.

  Changes in miRNA expression profile of space-flown Caenorhabditis elegans during Shenzhou-8 mission.Life Sciences in Space Research.2014.

  Anti-inflammatory effect and prostate gene expression profiling of steryl ferulate on experimental rats with non-bacterial prostatitis.Food Funct.2014.

  The onset of human ectopic pregnancy demonstrates a differential expression of miRNAs and their cognate targets in the Fallopian tube. Int J Clin Exp Pathol.2014.

  Gallic acid induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 human chondrosarcoma cells.Int J Oncol.2014.

  Serum microRNAs as potential biomarkers of mandibular prognathism.Oral Diseases.2014.

  Androgen receptor decreases CMYC and KRAS expression by upregulating let-7a expression in ER-, PR-, AR+ breast cancer.Int J Oncol.2014.

  The Differential Expression of MicroRNAs Between Implantation Sites and Interimplantation Sites in Early Pregnancy in Mice and Their Potential Functions.Reproductive Sciences.2014.

  MicroRNA profiling in the serums of SCA3/MJD patients. Int.J.Neurosci.2014.

  Characterization of microRNA expression profiles in patients with intervertebral disc degeneration.Int J Mol Med.2014.

  Genome-wide microarray-based analysis of miRNAs expression in patients with , acute-on-chronic liver failure.Hepatobiliary Pancreat Dis Int.2014.

  Treatment with Rhizoma Dioscoreae Extract Has Protective Effect on Osteopenia in Ovariectomized Rats.The Scientific World JOURNAL.2014.

  Silencing Kif2a induces apoptosis in squamous cell carcinoma of the oral tongue through inhibition of the PI3K/Akt signaling pathway.Mol Med Rep.2014.

  Characterization of the MicroRNA Expression Profile of Cervical Squamous Cell Carcinoma Metastases.Asian Pac J Cancer Prev.2014.

→  Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress.Chinese Journal of Oceanology and Limnology.2014.

→  Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma. Hepatol Int. 2013.

→  Acarbose reduces blood glucose by activating miR-10a-5p and miR-664 in diabetic rats. PLoS One. 2013.

→  Differentially expressed miRNAs after GnRH treatment and their potential roles in FSH regulation in porcine anterior pituitary cell. PLoS One. 2013.

→  Clinicopathologic significance of miR-10b expression in gastric carcinoma. Hum Pathol. 2013.

→  MicroRNA-204-5p regulates epithelial-to-mesenchymal transition during human posterior capsule opacification by targeting SMAD4. Invest Ophthalmol Vis Sci. 2013.

→  Global microRNA expression profiling reveals differential expression of target genes in 6-hydroxydopamine-injured MN9D cells. Neuromolecular Med. 2013.

→  Anti-miRNA-23a Oligonucleotide Suppresses Glioma Cells Growth by Targeting Apoptotic Protease Activating Factor-1. Current Pharmaceutical Design. 2013.

→  Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Nature Immunology. 2013.

→  TGF-b1 suppression of microRNA-450b-5p expression: a novel mechanism for blocking myogenic differentiation of rhabdomyosarcoma. Oncogene. 2013.

→  Identification of recurrence-related microRNAs in hepatocellular carcinoma following liver transplantation. Molecular Oncology. 2013.

→  MicroRNA-140-5p Suppresses Tumor Growth and Metastasis by Targeting Transforming Growth Factor β Receptor 1 and Fibroblast Growth Factor 9 in Hepatocellular Carcinoma. Hepatology. 2013.

→  microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment. Oncol Rep. 20, 12.

→  Differential expression of microRNA patterns in planarian normal and regenerative tissues. Mol Biol Rep. 2012.

→  A panel of serum microRNAs as specific biomarkers for diagnosis of compound- and herb-induced liver injury in rats. PLoS One. 2012.

→  Identification of aberrantly expressed miRNAs in rectal cancer. Oncol Rep. 2012.

→  Altered expression profiles of microRNAs upon arsenic exposure of human umbilical vein endothelial cells. Environ Toxicol Pharmacol. 2012.

→  Altered microRNAs expression profiling in experimental silicosis rats. J TOXICOL SCI . 2012.

→  The MicroRNA-328 Regulates Hypoxic Pulmonary Hypertension by Targeting at Insulin Growth Factor 1 Receptor and L-Type Calcium Channel- 1C. Hypertensionaha. 2012.

→  miR-30c-1* promotes natural killer cell cytotoxicity against human hepatoma cells by targeting the transcription factor HMBOX1. Cancer science. 2012.

→  MicroRNA-494 Is Required for the Accumulation and Functions of Tumor-Expanded Myeloid-Derived Suppressor Cells via Targeting of PTEN. The Journal of Immunology. 2012

→  Glucocorticoids inhibit lipopolysaccharide-mediated inflammatory response by
downregulating microRNA-155: a novel anti-inflammation mechanism. Free Radical Biology & Medicine. 2012.

→  Suppression of Type I Collagen Expression by miR-29b via PI3K, Akt, and Sp1 Pathway in Human Tenon’s Fibroblasts. Glaucoma. 2012.

→  Identification of new aberrantly expressed miRNAs in intestinal-type gastric cancer and its clinical significance. Oncol Rep. 2011

→  Overexpression of miR-125b, a novel regulator of innate immunity, in eosinophilic chronic rhinosinusitis with nasal polyps. American Thoracic Society.Am. J. Respir. Crit. 2011.

→  Dysregulated expression of miR-146a contributes to agerelated dysfunction of macrophages. Anatomical Society of Great Britain and Ireland. 2011.

→  MicroRNA-135a contributes to the development of portal vein tumor thrombus by promoting metastasis in hepatocellular carcinoma. Journal of Hepatology. 2011.

→  Signature microRNA Expression Profile of Essential Hypertension and Its Novel Link to Human Cytomegalovirus Infection. Circulation. 2011.

→  Deregulated miR-155 promotes Fas-mediated apoptosis in human intervertebral disc degeneration by targeting FADD and caspase-3. J Pathol. 2011.

→  Micro, RNA 345, a methylation-sensitive microRNA is involved in cell proliferation and invasion in human colorectal cancer. Carcinogenesis. 2011.

→  Modulation of T cell cytokine production by miR-144* with elevated expression in patients with pulmonary tuberculosis. Molecular Immunology. 2011.

→  A microRNA profile comparison between thoracic aortic dissection and normal thoracic aorta indicates the potential role of microRNAs in contributing to thoracic aortic dissection pathogenesis. JOURNAL OF VAS CULAR, SURGERY. 2011.

→  Upregulation of Mir-21 Mediates Resistance to Trastuzumab Therapy for Breast Cancer. Journal of Biological Chemistry. 2011.

→  MicroRNA Expression Analysis: Clinical Advantage of Propranolol Reveals Key MicroRNAs in Myocardial Infarction. PLoS ONE. 2011.

→  MiRNA profile in esophageal squamous cell carcinoma: Downregulation of miR-143 and miR-145. World J Gastroenterol. 2011.

→  Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues. Int J Oral Sci. 2011.

→  TLR9 agonist enhances lung cancer invasiveness by alternating miRNA expression profile.  Scientific Research and Essays. 2011.

→  Identification of microRNA-target interaction in APRIL-knockdown colorectal cancer cells. Cancer Gene Therapy. 2011.

→  MicroRNA-2, 24 Is Involved in Transforming Growth Fa, ctor-β-Mediated Mouse Granulosa Cell Proliferation and Granulosa Cell Function by Targeting Smad4. J., Mol. Endocrinol. 2010.

→  MiR-218 Inhibits Invasion and Metastasis of Gastric Cancer by Targeting the Robo1 Receptor. Plos Genetics. 2010.

→  A Novel Function of MicroRNA Let-7d in Regulation of Galectin-3 Expression in Attention Deficit Hyperactivity Disorder Rat Brain. Brain Pathol. 2010.

→  miR-221/222 suppression protects against endoplasmic reticulum stress-induced apoptosis via p27Kip1-and MEK/ERK-mediated cell cycle regulation. Biological Chemistry. 2010.

→  Up-regulation of microRNA in bladder tumor tissue is not common. Int Urol Nephrol. 2010.

→  MicroRNA-125b Confers the Resistance of Breast Cancer Cells to Paclitaxel through Suppression of Pro-apoptotic Bcl-2 Antagonist Killer 1 (Bak1) Expression. J Biol Chem. 2010.

→  Regulation of NADPH Oxidase Activity Is Associated with miRNA-25-Mediated NOX4 Expression in Experimental Diabetic Nephropathy. American Journal of Nephrology. 2010.

→  Differential expression of microRNAs in porcine placentas on Days 30 and 90 of gestation.   Reproduction, Fertility and Development. 2010.

→  MicroRNA profiling in mid- and late-gestational fetal skin: implication for scarless wound healing. Tohoku J Exp Med. 2010.

→  differential expression profiles of microRNAs in NIH3T3 Cells in Response to UVB Irradiation. Photochemistry and Photobiology. 2009.

→  Altered microRNA expression in patients with non-obstructive azoospermia. Reprod. Biol. Endocrinol. 2009.

→  Regulation of Hepatic MicroRNA Expression in Response to Ischemic Preconditioning following Ischemia=Reperfusion Injury in Mice. OMICS A Journal of Integrative Biology. 2009.

→  MicroRNA: Novel Regulators Involved in the Remodeling and Reverse Remodeling of the Heart. Cardiology. 2009.

→  Repression of the miR-17-92 cluster by p53 has an important function in hypoxia-induced apoptosis. EMBO J. 2009.

→  miR-15b and miR-16 are implicated in activation of the rat hepatic stellate cell: An essential role for apoptosis. J Hepatol. 2009.

→  Changes in microRNAs associated with hepatic stellate cell activation status identify signaling pathways. FEBS J. 2009.

→  Embryonic stem cells derived from somatic cloned and fertilized blastocysts are post-transcriptionally indistinguishable: A MicroRNA and protein profile comparison.  Proteomics. 2009.

→  Altered gene expression and miRNA expression associated with cancerous IEC-6 cell transformed by MNNG. J Exp Clin Canc Res. 2009.

→  Differential microRNA expression in childhood B-CELL precursor acute lymphoblastic leukemia. Pediatric Hematology-Oncology. 2009.

→  Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues.  J CANCER RES CLIN. 2009.

→  Changes in microRNA profile and effects of miR-320 in insulin-resistant 3T3-L1 adipocytes. CLIN EXP PHARMACOL P. 2009.

→  MicroRNA expression and regulation in mouse uterus during embryo implantation. J Biol Chem. 2008.

→  Microarray analysis of microRNA expression in hepatocellular carcinoma and non-tumorous tissues without viral hepatitis. J Gastroen Hepatol. 2008.

→  Identification of Differentially Expressed MicroRNAs During the Development of Chinese Murine Mammary Gland. Journal of Genetics and Genomics. 2007.


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